CLCN3 antibody (AA 98-115) (Atto 390)

Catalog No. ABIN2485470

This Mouse Monoclonal antibody specifically detects CLCN3 in WB, IHC, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for CLCN3 antibody (AA 98-115) (Atto 390) (ABIN2485470)

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLCN3 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S258-5

Product Details anti-CLCN3 Antibody (Atto 390)

Binding Specificity: AA 98-115

Specificity: Detects ~90 kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485470 was sufficient for detection of Clcn3 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for CLCN3

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Alternative Name: ClCN3

Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.

Gene ID: 84360

NCBI Accession: NP_445815

UniProt: P51792