KCNJ8 antibody (AA 306-424) (Atto 594)

Catalog No. ABIN2485703

The Atto 594-conjugated Mouse Monoclonal anti-KCNJ8 antibody (Clone S366-60) (ABIN2485703) specifically detects KCNJ8 in WB, IHC, IF and ICC. The antibody is reactive with Rat samples.

Quick Overview for KCNJ8 antibody (AA 306-424) (Atto 594) (ABIN2485703)

Target: KCNJ8 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 8 (KCNJ8))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNJ8 antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S366-60

Product Details anti-KCNJ8 Antibody (Atto 594)

Binding Specificity: AA 306-424

Specificity: Detects ~45 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:100 dilution of ABIN2485703 was sufficient for detection of Kir6.1 in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for KCNJ8

Target: KCNJ8 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 8 (KCNJ8))

Alternative Name: Kir6.1

Background: Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family (1-3). This is predominantly detected in fetal and adult hearts, and defects can be associated with J-wave syndromes, a group of heart disorders characterized by early repolarization events (4).

Gene ID: 25472

NCBI Accession: NP_058795

UniProt: Q63664