GABRA1 antibody (Cytoplasmic Domain) (Atto 390)

Catalog No. ABIN2485753

The Mouse Monoclonal anti-GABRA1 antibody is suitable to detect GABRA1 in samples from Human. It has been validated for WB, IHC, ICC and IF.

Quick Overview for GABRA1 antibody (Cytoplasmic Domain) (Atto 390) (ABIN2485753)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABRA1 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S415-24

Product Details anti-GABRA1 Antibody (Atto 390)

Binding Specificity: AA 368-419, Cytoplasmic Domain

Specificity: Detects ~55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:100 dilution of ABIN2485753 was sufficient for detection of GABA-A R, Alpha5 in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABA A Receptor

Background: The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).

Gene ID: 2558

NCBI Accession: NP_000801

UniProt: P31644