SLC12A3 antibody (AA 74-95) (Atto 390)

Catalog No. ABIN2486282

The Atto 390-conjugated Rabbit Polyclonal anti-SLC12A3 antibody (ABIN2486282) specifically detects SLC12A3 in WB, IHC, ICC, IEM and IF. The antibody is reactive with Rat samples.

Quick Overview for SLC12A3 antibody (AA 74-95) (Atto 390) (ABIN2486282)

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A3 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Immunofluorescence (IF)

Product Details anti-SLC12A3 Antibody (Atto 390)

Binding Specificity: AA 74-95

Specificity: Detects ~160 kDa.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Affinity Purified

Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2486282 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SLC12A3

Target: SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))

Alternative Name: NCC

Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Gene ID: 54300, 6559

NCBI Accession: NP_062218, NP_000330

UniProt: P55018, P55017