AQP3 antibody (C-Term) (Biotin)

Catalog No. ABIN2486360

This anti-AQP3 antibody is a Rabbit Polyclonal antibody detecting AQP3 in WB, IHC, IF and ICC. Suitable for Rat.

Quick Overview for AQP3 antibody (C-Term) (Biotin) (ABIN2486360)

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-AQP3 Antibody (Biotin)

Binding Specificity: C-Term

Specificity: Detects ~31.5 kDa. May detect larger glycosylated bands ~35-50 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2486360 was sufficient for detection of aquaporin 3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for AQP3

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Alternative Name: Aquaporin 3

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).

Gene ID: 65133

NCBI Accession: NP_113891

UniProt: P47862