AQP3 antibody (C-Term)

Catalog No. ABIN3044413

Product Details anti-AQP3 Antibody

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Binding Specificity: AA 278-292, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Aquaporin-3(AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: EENVKLAHVK HKEQI

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Aquaporin-3(AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: aquaporin 3(Gill blood group)
Protein Name: Aquaporin-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3(278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-AQP3 antibody (ABIN3044413)

Shin, Lee, Gil, Kim, Choe, Kim, Lee, Lim: "Agerarin, identified from Ageratum houstonianum, stimulates circadian CLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes." in: Scientific reports, Vol. 7, Issue 1, pp. 11175, (2017) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Target Details for AQP3

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Alternative Name: AQP3 (AQP3 Products)

Synonyms: aqp3 antibody, wu:fa95h06 antibody, wu:fi13e05 antibody, zgc:55525 antibody, Tb06.28P18.240 antibody, AQP-2 antibody, AQP-3 antibody, GIL antibody, SAQP3 antibody, aquaporin 3a antibody, aquaporin 3 (Gill blood group) antibody, aquaporin 3 antibody, aqp3a antibody, AQP3 antibody, Tb927.6.1520 antibody, MCYG_07750 antibody, Aqp3 antibody

Background: This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Synonyms: AQP 3 antibody|AQP-3 antibody|Aqp3 antibody|AQP3_HUMAN antibody|Aquaglyceroporin-3 antibody|Aquaporin 3(GIL blood group) antibody|Aquaporin 3(Gill blood group) antibody|Aquaporin-3 antibody|Aquaporin3 antibody|GIL antibody|Gill blood group antibody

UniProt: Q92482