Aquaporin 1 antibody (N-Term) (Atto 390)

Catalog No. ABIN2486434

The Rabbit Polyclonal anti-Aquaporin 1 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect Aquaporin 1 in samples from Rat.

Quick Overview for Aquaporin 1 antibody (N-Term) (Atto 390) (ABIN2486434)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Aquaporin 1 Antibody (Atto 390)

Binding Specificity: N-Term

Specificity: Detects ~28.5 kDa. May detect larger glycosylated bands ~35-50 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2486434 was sufficient for detection of aquaporin 1 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin 1

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).

Gene ID: 25240

NCBI Accession: NP_036910

UniProt: P29975

Pathways: Hormone Transport