CENPA antibody (PerCP)

Catalog No. ABIN2486499

This Mouse Monoclonal antibody specifically detects CENPA in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CENPA antibody (PerCP) (ABIN2486499)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPA antibody is conjugated to PerCP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5A7-2E11

Product Details anti-CENPA Antibody (PerCP)

Specificity: Detects ~18 kDa. Recognizes Human CENP-A.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Synthetic peptide corresponding to a portion of human CENP-A

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2486499 was sufficient for detection of CENPA in 20 μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA

Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Gene ID: 1058

NCBI Accession: NP_001035891

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location