P4HB antibody (AA 409-509) (Atto 390)

Catalog No. ABIN2486765

This anti-P4HB antibody is a Rabbit Polyclonal antibody detecting P4HB in WB, IHC, IP, IF and ICC. Suitable for Rat.

Quick Overview for P4HB antibody (AA 409-509) (Atto 390) (ABIN2486765)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-P4HB Antibody (Atto 390)

Binding Specificity: AA 409-509

Specificity: Detects ~58 kDa.

Cross-Reactivity: Cow, Dog, Guinea Pig, Hamster, Human, Mollusca, Mouse, Pig, Rat, Sheep, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogen: Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2486765 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI

Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).

Gene ID: 287164

NCBI Accession: NP_001099245

UniProt: P04785

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism