COL3A1 antibody (Chain alpha 1)

Catalog No. ABIN7581223

This Rabbit Polyclonal antibody specifically detects COL3A1 in WB, IF, IHC (p), IHC (fro), EIA and RIA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for COL3A1 antibody (Chain alpha 1) (ABIN7581223)

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Product Details anti-COL3A1 Antibody

Binding Specificity: Chain alpha 1

Specificity: Rat Collagen type III 100 % . Rat Collagen I < 0.1 % Rat Collagen type V < 10 % Rat Elastin < 0.1 % (determined by solid phase RIA at 1/50 dilution).

Purification: Affinity Chromatography

Immunogen: Purified Collagen type III from Rat skin.

Application Details

Application Notes: RIA. Western blot. ELISA: >/= 1/200. Immunflourescence: 1/80. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: >/= 1/500. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 minutes at RT or 2-8 °C over night. Positive Control: Rat skin or liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water. For further dilutions use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA or preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.Synonyms: COL3A1

Gene ID: 12825

NCBI Accession: NP_034060

UniProt: P08121

Pathways: Autophagy, Growth Factor Binding