14.3.3-Like Protein C (14.3.3C) antibody

Catalog No. ABIN249336

Product Details

Target: 14.3.3-Like Protein C (14.3.3C)

Reactivity: Barley

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Horderum vulgare

Characteristics: Expected / apparent Molecular Weight of the Antigene: 30 kDa

Purification: serum

Immunogen: synthetic peptide specific to the barley C isoform, conjugated to KLH

Application Details

Application Notes: 1: 2000 with standard ECL (WB), 1: 15 000 (ELISA)

Comment:

specific for barley 14.3.3C isoform, when tested against 14.3.3A, B and C isoforms

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References

Pertl, Pöckl, Blaschke, Obermeyer: "Osmoregulation in Lilium pollen grains occurs via modulation of the plasma membrane H+ ATPase activity by 14-3-3 proteins." in: Plant physiology, Vol. 154, Issue 4, pp. 1921-8, (2010) (PubMed).

Target Details

Target: 14.3.3-Like Protein C (14.3.3C)

Background: 14.3.3's are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3's include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.

Molecular Weight: 30 kDa