Nucleaoside Diphosphate Kinase antibody

Catalog No. ABIN249380

The Rabbit Polyclonal anti-Nucleaoside Diphosphate Kinase antibody has been validated for WB. It is suitable to detect Nucleaoside Diphosphate Kinase in samples from Arabidopsis thaliana and Pisum sativum.

Quick Overview for Nucleaoside Diphosphate Kinase antibody (ABIN249380)

Target: Nucleaoside Diphosphate Kinase (NDPK)

Reactivity: Arabidopsis thaliana, Pisum sativum

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Nucleaoside Diphosphate Kinase Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Brassica campestris, Spinacia oleracea, Vitis vinifera, monocots indlucing: Oryza sativa

Characteristics: Expected / apparent Molecular Weight of the Antigene: 25 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.

Application Details

Application Notes: 1: 5000 (WB)

Comment:

1: 5000 dilution in a western blot was used for 15 µg of protein per lane

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Nucleaoside Diphosphate Kinase

Target: Nucleaoside Diphosphate Kinase (NDPK)

Alternative Name: NDPK

Background: AGI Code: At4g11010
Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.

Molecular Weight: 25 kDa

UniProt: Q9SP13