Sar1 antibody

Catalog No. ABIN7477852

Cited in 3+ publications. The Rabbit Polyclonal anti-Sar1 antibody (ABIN7477852) specifically detects Sar1 in EM and WB. The antibody is reactive with Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis neomexicana, Megathyrsus maximus, Petunia hybrida, Proso millet (Panicum miliaceum), Pteris vittata (Chinese ladder brake), Triticum aestivum and Zea mays samples.

Quick Overview for Sar1 antibody (ABIN7477852)

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Reactivity: Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis neomexicana, Megathyrsus maximus, Petunia hybrida, Proso millet (Panicum miliaceum), Pteris vittata (Chinese ladder brake), Triticum aestivum, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Electron Microscopy (EM), Western Blotting (WB)

Product Details anti-Sar1 Antibody

Purpose: Anti-Sar1 | Secretion-associated and Ras-related protein 1

Cross-Reactivity (Details): Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
Not reactive in: Nicotiana tabacum

Predicted Reactivity: Citrus clementina,Medicago truncatula,Brassica rapa,Hordeum vulgare,Solanum tuberosum,Solanum lycopersicum,Populus trichocarpa,Glycine max,Vitis vinifera,Capsella rubella,Oryza sativa,Sorghum bicolor,Brachypodium distachyon,Brassica campestris,Brassica napus,Theobroma cacao,Triticum aestivum,Ricinus communis

Purification: Serum

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7

Application Details

Application Notes: 1 : 50 (IG), 1 : 500 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Sar1 antibody (ABIN7477852)

Liu, Fan, Li, Song, Li, Zhang, Xue: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Tnani, López, Jouenne, Vicient: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, Vol. 168, Issue 5, pp. 510-3, (2011) (PubMed).

Target Details for Sar1

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Background:

Background: Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Additional information: For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.

Molecular Weight: 21 kDa (Arabidopsis thaliana)

Gene ID: 825430

UniProt: Q8VYP7