GLN1 antibody (all Isoforms)

Catalog No. ABIN2559393

The Rabbit Polyclonal anti-GLN1 antibody has been validated for WB. It is suitable to detect GLN1 in samples from Arabidopsis thaliana, Spinach and Zea mays. There is 1 publication available.

Quick Overview for GLN1 antibody (all Isoforms) (ABIN2559393)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Arabidopsis thaliana, Spinach, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLN1 Antibody

Binding Specificity: all Isoforms

Specificity: The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1###-1,1-###2,1-###3 and 1-4, (At5g37600, At5g66200, At5g17820, At5g16570)

Application Details

Application Notes: Recommended Dilution: 1 : 10000 on 0.5-15 ug protein (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-GLN1 antibody (ABIN2559393)

Ortigosa, Valderrama-Martín, Ávila, Cánovas, Cañas: "Understanding plant nitrogen nutrition through a laboratory experiment." in: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, Vol. 47, Issue 4, pp. 450-458, (2019) (PubMed).

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: GS1 glutamine synthetase

Background: AGI Code: At5g37600
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts

Molecular Weight: 39-40 kDa (GLN1 kDa,cytoplasmic form) , 44-45 kDa (GLN2 kDa, chloroplastic form)

UniProt: Q56WN1, Q9FMD9, Q8LCE1, Q9LVI8

Pathways: Positive Regulation of Peptide Hormone Secretion