DTX3L antibody (AA 651-740) (Biotin)

Catalog No. ABIN2559609

The Rabbit Polyclonal anti-DTX3L antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect DTX3L in samples from Human, Mouse and Rat.

Quick Overview for DTX3L antibody (AA 651-740) (Biotin) (ABIN2559609)

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DTX3L antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DTX3L Antibody (Biotin)

Binding Specificity: AA 651-740

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DTX3L

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for DTX3L

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Alternative Name: DTX3L

Background:

Synonyms: BBAP, E3 ubiquitin-protein ligase DTX3L, B-lymphoma- and BAL-associated protein, Protein deltex-3-like, Rhysin-2, Rhysin2, DTX3L

Background: Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

Gene ID: 151636

UniProt: Q8TDB6

Pathways: Notch Signaling