DGKH antibody (AA 51-150)

Catalog No. ABIN2559726

The Rabbit Polyclonal anti-DGKH antibody has been validated for ELISA, IHC (p), IHC (fro), ICC, IF (cc) and IF (p). It is suitable to detect DGKH in samples from Human and Rat.

Quick Overview for DGKH antibody (AA 51-150) (ABIN2559726)

Target: DGKH (Diacylglycerol Kinase, eta (DGKH))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKH antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DGKH Antibody

Binding Specificity: AA 51-150

Predicted Reactivity: Mouse,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DGKH

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DGKH

Target: DGKH (Diacylglycerol Kinase, eta (DGKH))

Alternative Name: DGKH

Background:

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

Subcellular location: Cytoplasm

Synonyms: DGKeta, Diacylglycerol kinase eta, DAG kinase eta, Diglyceride kinase eta, DGK-eta, DGKH

Gene ID: 160851

UniProt: Q86XP1