The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
PLD1 (phospho Thr147) antibody was raised against a peptide sequence around phosphorylation site of threonine 147 (R-H-T (p)-F-R) derived from Human PLD1.
PLD1
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Western Blot: 1:500~1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store antibody at -20°C for up to one year.
Target
PLD1
(Phospholipase D1 (PLD1))
Alternative Name
PLD1
Background
Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.