ACTN1 antibody (AA 596-609)

Catalog No. ABIN2613374

This Goat Polyclonal antibody specifically detects ACTN1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACTN1 antibody (AA 596-609) (ABIN2613374)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ACTN1 Antibody

Binding Specificity: AA 596-609

Purpose: ACTN1 (aa596-609)

Sequence: TPQEINGKWD HVRQ

Specificity: This antibody is expected to recognize all reported isoforms (NP_001123476.1, NP_001093.1, NP_001123477.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPQEINGKWDHVRQ, from the internal region of the protein sequence

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103 kDa according to NP_001093.1). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 75 kDa. Th

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: ACTN1, actinin, alpha 1, F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1

Gene ID: 87, 109711, 81634

NCBI Accession: NP_001123476, NP_001093, NP_001123477

Pathways: Cell-Cell Junction Organization