ErbB2/Her2 antibody (AA 182-194)

Catalog No. ABIN2613422

This Goat Polyclonal antibody specifically detects ErbB2/Her2 in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for ErbB2/Her2 antibody (AA 182-194) (ABIN2613422)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: AA 182-194

Purpose: ERBB2 / HER2 (aa182-194)

Sequence: TLIDTNRSRA CHP

Specificity: This antibody is expected to recognize both reported isoforms (NP_004439.2, NP_001005862.1). The immunizing peptide represents part of the extracellular domain.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration, 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Background: ERBB2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, c-erb B2/neu protein, herstatin, metastatic lymph node gene 19 protein, neuroblas

Gene ID: 2064

NCBI Accession: NP_004439, NP_001005862

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development