Nanog antibody (AA 270-284)

Catalog No. ABIN2613457

This anti-Nanog antibody is a Goat Polyclonal antibody detecting Nanog in WB and ELISA. Suitable for Mouse.

Quick Overview for Nanog antibody (AA 270-284) (ABIN2613457)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Nanog Antibody

Binding Specificity: AA 270-284

Purpose: Nanog (mouse aa270-284)

Sequence: DLEVNLEATR ESHAH

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 34.6 kDa according to NP_082292.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zbinden et al, EMBO J. 201

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Background: Nanog, Nanog homeobox, 2410002E02Rik, ENK, ecat4, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox protein NANOG, homeobox transcription factor Nanog

Gene ID: 71950

NCBI Accession: NP_082292

Pathways: Stem Cell Maintenance