Peripherin antibody (C-Term)

Catalog No. ABIN2613465

The Goat Polyclonal anti-Peripherin antibody has been validated for WB, IHC and ELISA. It is suitable to detect Peripherin in samples from Rat and Mouse. There is 1 publication available.

Quick Overview for Peripherin antibody (C-Term) (ABIN2613465)

Target: Peripherin (PRPH)

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Peripherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Peripherin Antibody

Binding Specificity: C-Term

Purpose: Peripherin 1 (mouse)

Sequence: QKEQHSDLDK SSIH

Specificity: This antibody is expected to recognize all reported isoforms (NP_038667.2, NP_001157060.1, NP_001157061.1).

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody has been successfully used in IHC on Mouse, PMID: 36226085.

Western Blot: Approx 55 kDa band observed in Rat Colon lysates (calculated MW of 54.0 kDa according to NP_036765.2). Recommended concentration: 0.1-0.3 μg/mL. An additional faint band of unknown identity was also consistently observed at 75 kDa. This band wa

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Peripherin antibody (ABIN2613465)

Froud, Wong, Cederholm, Klugmann, Sandow, Julien, Ryan, Housley: "Type II spiral ganglion afferent neurons drive medial olivocochlear reflex suppression of the cochlear amplifier." in: Nature communications, Vol. 6, pp. 7115, (2016) (PubMed).

Target Details for Peripherin

Target: Peripherin (PRPH)

Alternative Name: Prph

Background: Prph, peripherin, Prph1, peripherin 1

Gene ID: 19132, 24688

NCBI Accession: NP_038667, NP_001157060, NP_001157061