SLC40A1 antibody (AA 245-259)

Catalog No. ABIN2613478

The Goat Polyclonal anti-SLC40A1 antibody (ABIN2613478) specifically detects SLC40A1 in ELISA, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for SLC40A1 antibody (AA 245-259) (ABIN2613478)

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC40A1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-SLC40A1 Antibody

Binding Specificity: AA 245-259

Purpose: SLC40A1 (aa245-259)

Sequence: ELKQLNLHKD TEPKP

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 60 kDa in Human Breast and Colonr lysate after 1-2 μg/mL antibody staining (calculated MW of 62.5 kDa according to NP_055400.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended con

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SLC40A1

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Alternative Name: SLC40A1

Background: SLC40A1, solute carrier family 40 (iron-regulated transporter), member 1, FPN1, HFE4, IREG1, MST079, MTP1, SLC11A3, iron regulated gene 1, putative ferroportin 1 variant IIIB, solute carrier family 11 (proton-coupled divalent metal ion transporters), memb

Gene ID: 30061

NCBI Accession: NP_055400

Pathways: Transition Metal Ion Homeostasis