CASP14 antibody (AA 223-242)

Catalog No. ABIN2617926

The Rabbit Polyclonal anti-CASP14 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CASP14 in samples from Human, Mouse and Rat.

Quick Overview for CASP14 antibody (AA 223-242) (ABIN2617926)

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CASP14 Antibody

Binding Specificity: AA 223-242

Specificity: Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846). .

Cross-Reactivity (Details): No cross reactivity with other proteins.

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14(223-242aa KARKTNPEIQSTLRKRLYLQ), different from the related rat and mouse sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide per 100 μg antibody.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.

Target Details for CASP14

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: CASP14 / Caspase 14

Background: Name/Gene ID: CASP14
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP14, Caspase-14, Caspase 14, MICE, CASP-14

Gene ID: 23581