Cytokeratin 18 antibody (AA 265-276)

Catalog No. ABIN2624658

The Goat Polyclonal anti-Cytokeratin 18 antibody has been validated for WB, IHC, ELISA and IHC (p). It is suitable to detect Cytokeratin 18 in samples from Human, Mouse, Rat, Pig, Cow, Horse, Monkey, Rabbit, Guinea Pig, Xenopus laevis and Bat.

Quick Overview for Cytokeratin 18 antibody (AA 265-276) (ABIN2624658)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Monkey, Rabbit, Guinea Pig, Xenopus laevis, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: AA 265-276

Specificity: Human KRT18 / Cytokeratin 18. Reported variants represent identical protein: NP_000215.1, NP_954657.1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EELDKYWSQQIE from an internal region of human KRT18 / Cytokeratin 18 (NP_000215.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Ferret, Bovine, Bat, Elephant, Panda, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%), Platypus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kD according to NP_000215.1). Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18 / CK18 / Cytokeratin 18

Background: Name/Gene ID: KRT18
Family: Keratin

Synonyms: KRT18, CK-18, Cytokeratin 18, Cytokeratin-18, K18, Keratin 18, CYK18, Keratin-18

Gene ID: 3875

NCBI Accession: NP_000215

Pathways: Apoptosis, Caspase Cascade in Apoptosis