MADCAM1 antibody
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- Target See all MADCAM1 Antibodies
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MADCAM1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
- Purification
- Protein G purified
- Endotoxin Level
- Low Endotoxin
- Immunogen
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Recombinant soluble MAdCAM-1-fc fusion protein.
Type of Immunogen: Fusion protein - Clone
- 314G8
- Isotype
- IgG1
- Top Product
- Discover our top product MADCAM1 Primary Antibody
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- Application Notes
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Approved: ELISA, Flo (1:10 - 1:100), IHC, IHC-P (10 μg/mL), WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections. Western Blot: LS-C44686 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
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- Target
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- Alternative Name
- MADCAM1 / MAdCAM-1 (MADCAM1 Products)
- Synonyms
- MACAM1 antibody, AV211525 antibody, mucosal vascular addressin cell adhesion molecule 1 antibody, MADCAM1 antibody, Madcam1 antibody
- Background
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Name/Gene ID: MADCAM1
Synonyms: MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1 - Gene ID
- 8174
- UniProt
- Q13477
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