MED1 antibody (C-Term)

Catalog No. ABIN2625326

This Rabbit Polyclonal antibody specifically detects MED1 in WB, IHC, IF, IP, IHC (p) and PLA. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Cow, Dog, Horse and Rabbit.

Quick Overview for MED1 antibody (C-Term) (ABIN2625326)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-MED1 Antibody

Binding Specificity: AA 1525-1581, C-Term

Brand: IHC-plus™

Specificity: Region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1 (Thyroid Hormone Receptor-Associated Protein Complex Component TRAP220) using the numbering given in entry NP_004765.2 (GeneID 54

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (5 μg/mL), IP (2 - 5 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1 / TRAP220

Background: Name/Gene ID: MED1

Synonyms: MED1, ARC205, CRSP1, DRIP205, DRIP230, Mediator complex subunit 1, PBP, PPARG binding protein, RB18A, TRIP-2, TRIP2, PPARBP, CRSP200, p53 regulatory protein RB18A, PPAR binding protein, PPAR-binding protein, PPARGBP, TR-interacting protein 2, TRAP220

Gene ID: 5469

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha