AIRE antibody (Internal Region)

Catalog No. ABIN263154

The Goat Polyclonal anti-AIRE antibody (ABIN263154) specifically detects AIRE in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for AIRE antibody (Internal Region) (ABIN263154)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-AIRE Antibody

Binding Specificity: Internal Region

Purpose: AIRE

Sequence: KAKPPKKPES SAEQ

Cross-Reactivity: Cow, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KAKPPKKPESSAEQ, from the internal region of the protein sequence according to NP_000374.1, NP_000649.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in human spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background: AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), a

Gene ID: 326, 11634

NCBI Accession: NP_000374

Pathways: Chromatin Binding