FMR1 antibody (Internal Region)

Catalog No. ABIN263156

The Goat Polyclonal anti-FMR1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect FMR1 in samples from Human.

Quick Overview for FMR1 antibody (Internal Region) (ABIN263156)

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-FMR1 Antibody

Binding Specificity: Internal Region

Purpose: FMR1

Sequence: RTGKDRNQKK EKPD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C Terminus) of the protein sequence according to NP_002015.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell line KELLY (calculated MW of 71.2 kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Gene

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMR1

Background: FMR1, fragile X mental retardation 1 , FMRP, FRAXA, MGC87458

Gene ID: 2332

NCBI Accession: NP_002015

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development