ATG16L1 antibody (Internal Region)

Catalog No. ABIN263157

This anti-ATG16L1 antibody is a Goat Polyclonal antibody detecting ATG16L1 in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for ATG16L1 antibody (Internal Region) (ABIN263157)

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG16L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ATG16L1 Antibody

Binding Specificity: Internal Region

Purpose: ATG16L1

Sequence: KVEKVLSKQH SSSIN

Specificity: This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KVEKVLSKQHSSSIN

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 65 kDa band observed in Mouse Brain lysates (calculated MW of 68.1 kDa according to Mouse NP_084122.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: ATG16L1

Background: ATG16 , autophagy related 16-like 1 (S. cerevisiae) , hCG_1817841, APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, WDR30, APG16 autophagy 16-like, APG16L beta, ATG16 autophagy related 16-like protein 1, WD repeat domain 30

Gene ID: 55054

NCBI Accession: NP_110430, NP_060444

Pathways: Autophagy