Vitamin D-Binding Protein antibody (Internal Region)

Catalog No. ABIN263165

The Goat Polyclonal anti-Vitamin D-Binding Protein antibody has been validated for WB and ELISA. It is suitable to detect Vitamin D-Binding Protein in samples from Human. There is 1 publication available.

Quick Overview for Vitamin D-Binding Protein antibody (Internal Region) (ABIN263165)

Target: Vitamin D-Binding Protein (GC)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Vitamin D-Binding Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Vitamin D-Binding Protein Antibody

Binding Specificity: Internal Region

Purpose: Vitamin D-binding protein / GC

Sequence: CDNLSTKNSK FED

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CDNLSTKNSKFED, from the internal region of the protein sequence according to NP_000574.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 56 kDa band observed in Human Lung lysates (calculated MW of 52.9 kDa according to NP_000574.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (DiMartino and Kew, J Immunol. 1999 Aug 15,163(4):2135-42, PMID: 10438954). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Vitamin D-Binding Protein antibody (ABIN263165)

Vicente-Vicente, Ferreira, González-Buitrago, López-Hernández, López-Novoa, Morales: "Increased urinary excretion of albumin, hemopexin, transferrin and VDBP correlates with chronic sensitization to gentamicin nephrotoxicity in rats." in: Toxicology, Vol. 304, pp. 83-91, (2013) (PubMed).

Target Details for Vitamin D-Binding Protein

Target: Vitamin D-Binding Protein (GC)

Alternative Name: Vitamin D-binding protein / GC

Background: GC, vitamin D-binding protein, group-specific component (vitamin D binding protein), DBP, DBP/GC, VDBG, VDBP, vitamin D binding protein, vitamin D-binding alpha-globulin, vitamin D-binding protein/group specific component

Gene ID: 2638

NCBI Accession: NP_000574

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process