GPR125 antibody (Internal Region)

Catalog No. ABIN263209

This Goat Polyclonal antibody specifically detects GPR125 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for GPR125 antibody (Internal Region) (ABIN263209)

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GPR125 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GPR125 Antibody

Binding Specificity: Internal Region

Purpose: GPR125

Sequence: CTNKSASSFK NS

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL. A customer reported successful results on Human Testis.

Western Blot: Approx 140 kDa band observed in Human Ovary lysates (calculated MW of 146 kDa according to NP_660333.2). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently ob

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GPR125

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Alternative Name: ADGRA3

Background: GPR125 , G protein-coupled receptor 125 , PGR21, TEM5L, TEM5-like

Gene ID: 166647, 70693

NCBI Accession: NP_660333