HCRTR1 antibody (Internal Region)

Catalog No. ABIN263226

This anti-HCRTR1 antibody is a Goat Polyclonal antibody detecting HCRTR1 in ELISA and WB. Suitable for Human, Rat and Mouse.

Quick Overview for HCRTR1 antibody (Internal Region) (ABIN263226)

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HCRTR1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-HCRTR1 Antibody

Binding Specificity: Internal Region

Purpose: OX1R and OX2R

Sequence: YNFLSGKFRE QFK

Specificity: This antibody is expected to recognise both the human proteins OX1R and OX2R.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YNFLSGKFREQFK, from the internal region of the protein sequence according to NP_001516.2, NP_001517.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5 kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HCRTR1

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Alternative Name: HCRTR1

Background: OX1R, OX2R, HCRTR1, hypocretin (orexin) receptor 1, hypocretin receptor 1, hypocretin receptor-1, orexin receptor 1, orexin receptor-1, HCRTR2, hypocretin (orexin) receptor 2, hypocretin receptor 2, hypocretin receptor-2, orexin receptor 2, orexin recepto

Gene ID: 3061, 3062, 230777

NCBI Accession: NP_001516, NP_001517

Pathways: Feeding Behaviour