ABCA2 antibody (Internal Region)

Catalog No. ABIN263228

The Goat Polyclonal anti-ABCA2 antibody is suitable to detect ABCA2 in samples from Human. It has been validated for ELISA and IHC.

Quick Overview for ABCA2 antibody (Internal Region) (ABIN263228)

Target: ABCA2 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 2 (ABCA2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ABCA2 Antibody

Binding Specificity: Internal Region

Purpose: ABCA2

Sequence: KKQSDNLEQQ ETEP

Specificity: This antibody is expected to recognize both reported isoforms (NP_001597.2, NP_997698.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KKQSDNLEQQETEP, from the internal region of the protein sequence according to NP_001597.2, NP_997698.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 35 kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literatu

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ABCA2

Target: ABCA2 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 2 (ABCA2))

Alternative Name: ABCA2

Background: ABCA2, ATP-binding cassette, sub-family A (ABC1), member 2, ABC2, MGC129761, ATP-binding cassette, sub-family A, member 2, OTTHUMP00000064733

Gene ID: 20, 11305, 79248

NCBI Accession: NP_001597, NP_997698