APOA1 antibody

Catalog No. ABIN263693

The Mouse Monoclonal anti-APOA1 antibody is suitable to detect APOA1 in samples from Human. It has been validated for RIA and EIA.

Quick Overview for APOA1 antibody (ABIN263693)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: 057-10029

Product Details anti-APOA1 Antibody

Specificity: Recognizes Apolipoprotein A I. This antibody does not cross react with Apo AII or Apo B in competitive RIA.Aff. Const.:5 x 10e6 L/m (determined by RIA followed by Scatchard plot analysis)

Characteristics: Synonyms: APOA1, ApoA-I, Apo-AI, ApoAI

Purification: Protein A chromatography

Purity: > 90 % pure (SDS-PAGE)

Immunogen: Human high density lipoprotein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 5.3 mg/mL (OD280nm, E0.1% = 1.4)

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride and 0,09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one week or (in aliquots) at -40 °C for longer. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of thenominal fill volume of the vial used. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apolipoprotein A I (APO AI)

Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gene ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism