GAPDH antibody

Catalog No. ABIN263739

The Mouse Monoclonal anti-GAPDH antibody (Clone B903M) (ABIN263739) specifically detects GAPDH in WB, IF and EIA. The antibody is reactive with Human, Rat, Rabbit, Pig, Cow and Goat samples.

Quick Overview for GAPDH antibody (ABIN263739)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Rat, Rabbit, Pig, Cow, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: B903M

Product Details anti-GAPDH Antibody

Specificity: This antibody reacts with GAPDH. Does not react with GAPDH from Dog, Cat, Mouse and Fish.

Characteristics: Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase

Purification: Protein A Chromatography

Immunogen: Human or rabbit GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells fromBALB/c mice.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Immunocytochemistry. Recommended pairs for Sandwich Immunoassay: Capture: ABIN263741Detection: ABIN263739
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 4.5 mg/mL (Sigma protein assay kit)

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36 kDa subunits.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Gene ID: 2597

UniProt: P04406