GAPDH antibody

Catalog No. ABIN263741

The Mouse Monoclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human, Rabbit, Pig, Dog, Cat and Goat. It has been validated for EIA.

Quick Overview for GAPDH antibody (ABIN263741)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Rabbit, Pig, Dog, Cat, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: 6F7

Product Details anti-GAPDH Antibody

Specificity: This antibody recognizes GAPDH monomer (36 kDa) and the dimer form and does not react with the tetrameric form. Does not react with GAPDH from Bovine, Rat, Mouse and Fish.

Characteristics: Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase

Purification: Protein A Chromatography

Immunogen: Rabbit muscle GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/cmice.

Isotype: IgG1

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 4.1 mg/mL (Sigma protein assay kit)

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Gene ID: 2597

UniProt: P04406