OCT4 antibody

Catalog No. ABIN263988

This anti-OCT4 antibody is a Mouse Monoclonal antibody detecting OCT4 in IHC (p). Suitable for Human.

Quick Overview for OCT4 antibody (ABIN263988)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NRG1-1

Product Details anti-OCT4 Antibody

Specificity: OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-bindingtranscription factor 3, Oct-3, Oct-4

Purification: Purified

Immunogen: BALB/C mice injected with synthetic peptide of human OCT-4 origin.

Isotype: IgG2b

Application Details

Application Notes: Suitable for Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/100 in an ABC method. Positive Control: Seminoma. Cellular Localization: Nuclear.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Staining ProtocolWe suggest an incubation period of 30 minutes at room temperature. However, dependingupon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require

Restrictions: For Research Use only

Handling

Format: Liquid

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: OCT3/4

Background: OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance