CD3 antibody (APC)

Catalog No. ABIN264182

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Human.

Quick Overview for CD3 antibody (APC) (ABIN264182)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: M2AB

Product Details anti-CD3 Antibody (APC)

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood,Characterization of subtypes of T cell leukemia's and lymphomas, Studies of AIDS/HIVvirus infection, Analysis of CD3 complex related to the T cell antigen receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.08 % sodium azide as preservative and 0.2 % protein carrier.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85 % of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.W. surface antigen.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway