CD5 antibody (APC)

Catalog No. ABIN264188

The Mouse Monoclonal anti-CD5 antibody has been validated for FACS. It is suitable to detect CD5 in samples from Human.

Quick Overview for CD5 antibody (APC) (ABIN264188)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: M28323

Product Details anti-CD5 Antibody (APC)

Characteristics: Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of Bcell subsets, Characterization of subtypes of T cell leukemias and lymphomas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85 % peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 921

UniProt: P06127