CD33 antibody (PE)

Catalog No. ABIN264253

This Mouse Monoclonal antibody specifically detects CD33 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD33 antibody (PE) (ABIN264253)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: FOS

Product Details anti-CD33 Antibody (PE)

Specificity: This antibody recognises human Myeloid.

Characteristics: Synonyms: Siglec-3, gp67, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Characterization of subtypes of leukemias and lymphomas. Studies of AIDS virus infection. Myeloid cell function studies. Analysis of hematopoietic maturation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD33

Target: CD33

Alternative Name: CD33 / SIGLEC3

Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is also found on CFU-mix, CFU-GM, CFU-Meg, a portion of BFU-E, myeloblasts, promyelocytes, myelocytes, metamyelocytes but not early precursors.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID: 945

UniProt: P20138