CD42a antibody (FITC)

Catalog No. ABIN264318

Product Details anti-CD42a Antibody (FITC)

Target: CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42a antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises human Platelet GPIX.

Characteristics: Synonyms: Platelet glycoprotein IX, GP-IX, GP9, Glycoprotein 9

Clone: ESS

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Identification of platelets. Identification of megakaryocytes. Diagnosis of Bernard-Soulier syndrome (CD42a-). Megakaryoblastic/cytic leukemia's (CD42a+). See Protocols for more details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: UseConsult the appropriate Negative Control factsheet to determine the amount of antibody tobe used as a control for Platelets. Collect blood aseptically by venipuncture into an ACD or EDTA blood collection tube. Important: Within 5 minutes of blood collection, fix blood sample by placing 100 µl ofblood in test tube containing 1 ml of cold (4°C) 1X PBS with 1% paraformaldehyde. Mix by vortexing. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Prior to staining, wash the fixed blood pellet 2X with 1 ml 1X PBS + 0. 1% Azide at RT. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Resuspend the pellet in 1 ml of 1X PBS at RT. To a clean labeled test tube add 10 µl of MAb. Carefully add 50 ul of the fixed blood suspension to the bottom of the test tube. Vortex and incubate at room temperature for at least 15 minutes and analyzed within 3hours. Please note: Using this procedure will yield twenty samples for platelet analysis. Select logarithmic (Log) amplification for both Forward (FSC) and Side (SSC) scatters, whilecollecting data for platelets. See instrument manufacturer's instructions for Immunofluorescence analysis with a flowcytometer or microscope.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD42a

Target: CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))

Alternative Name: CD42a / GPIX (GP9 Products)

Background: Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW 23 kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.Synonyms: GP-IX, GP9, Glycoprotein 9, Platelet glycoprotein IX

Gene ID: 2815

UniProt: P14770