Calpain antibody (Agarose Beads)

Catalog No. ABIN264386

The Mouse Monoclonal anti-Calpain antibody has been validated for IP. It is suitable to detect Calpain in samples from Human.

Quick Overview for Calpain antibody (Agarose Beads) (ABIN264386)

Target: Calpain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calpain antibody is conjugated to Agarose Beads

Application: Immunoprecipitation (IP)

Clone: 28F3

Product Details anti-Calpain Antibody (Agarose Beads)

Characteristics: Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1

Purification: Standard chromatographic techniques.

Immunogen: Calpain purified from human placenta.

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Calpain

Target: Calpain

Background: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2