NAPA antibody

Catalog No. ABIN264390

The Mouse Monoclonal anti-NAPA antibody (Clone 16D1) (ABIN264390) specifically detects NAPA in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Cow and Chicken samples.

Quick Overview for NAPA antibody (ABIN264390)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 16D1

Product Details anti-NAPA Antibody

Specificity: The antibody recognizes an epitope within the highly conserved domain between amino acids 109 - 193 of SNAP-alpha and SNAP-beta.

Characteristics: Synonyms: SNAP-alpha, SNAPA, NAPA, Alpha-soluble NSF attachment protein

Purification: Standard chromatographic techniques.

Immunogen: Recombinant human alpha SNAP.

Isotype: IgG2b

Application Details

Application Notes: Suitable for use in Western Blot: 1/5000. Positive Control: Rat brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol, pH 7.5

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: SNAP alpha

Background: 38 kDa SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide- sensitive fusion protein (NSF) are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-toendosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. alpha-SNAP (or beta-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.Synonyms: Alpha-soluble NSF attachment protein, NAPA, SNAP-alpha, SNAPA

Gene ID: 8775

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization