NAPA antibody

Catalog No. ABIN264390

This anti-NAPA antibody is a Mouse Monoclonal antibody detecting NAPA in WB and EIA. Suitable for Human, Mouse, Rat, Cow and Chicken.

Quick Overview for NAPA antibody (ABIN264390)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 16D1

Product Details anti-NAPA Antibody

Specificity: The antibody recognizes an epitope within the highly conserved domain between amino acids 109 - 193 of SNAP-alpha and SNAP-beta.

Characteristics: Synonyms: SNAP-alpha, SNAPA, NAPA, Alpha-soluble NSF attachment protein

Purification: Standard chromatographic techniques.

Immunogen: Recombinant human alpha SNAP.

Isotype: IgG2b

Application Details

Application Notes: Suitable for use in Western Blot: 1/5000. Positive Control: Rat brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol, pH 7.5

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: SNAP alpha

Background: 38 kDa SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide- sensitive fusion protein (NSF) are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-toendosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. alpha-SNAP (or beta-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.Synonyms: Alpha-soluble NSF attachment protein, NAPA, SNAP-alpha, SNAPA

Gene ID: 8775

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization