NAPA antibody

Catalog No. ABIN264392

The Mouse Monoclonal anti-NAPA antibody has been validated for WB, IP and EIA. It is suitable to detect NAPA in samples from Human, Rat and Cow.

Quick Overview for NAPA antibody (ABIN264392)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D4

Product Details anti-NAPA Antibody

Specificity: This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.

Characteristics: Synonyms: NAPA, SNAP-alpha, SNAPA, Alpha-soluble NSF attachment protein,N-ethylmaleimide-sensitive factor attachment protein alpha

Purification: Standard chromatographic techniques.

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman a-SNAP protein and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5-5 μg/mL. Immunoprecipitationon native and denatured samples: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: SNAP alpha

Background: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.Synonyms: Alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, NAPA, SNAP-alpha, SNAPA

Gene ID: 8775

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization