RIT1 antibody

Catalog No. ABIN264412

This Mouse Monoclonal antibody specifically detects RIT1 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for RIT1 antibody (ABIN264412)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 14G7

Product Details anti-RIT1 Antibody

Specificity: This antibody detects recombinant Rit protein expressed in 293 cells. Does not cross-react with Rin protein.

Characteristics: Synonyms: RIBB, RIT, ROC1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein1

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinantfull-length human Rit protein and mouse myeloma cells. Genename: RIT1

Isotype: IgG1

Application Details

Application Notes: Western Blot (0.5-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium phosphate, 150 mM Sodium Chloride, 50 % Glycerol at pH 7.5 and 3 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: RIT1

Background: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: RIBB, RIT, ROC1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1

Gene ID: 6016

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway