RIT2 antibody

Catalog No. ABIN264413

The Mouse Monoclonal anti-RIT2 antibody is suitable to detect RIT2 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for RIT2 antibody (ABIN264413)

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RIT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 27G2

Product Details anti-RIT2 Antibody

Specificity: This antibody detects recombinant Rin protein expressed in 293 cells. Does not cross-react with Rit protein.

Characteristics: Synonyms: RIN, ROC2, GTP-binding protein Rit2, Ras-like protein expressed in neurons, Ras-likewithout CAAX protein 2

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full-lengthrecombinant human Rin protein and mouse myeloma cells. Genename: RIT2

Isotype: IgG2b

Application Details

Application Notes: Western Blot (0.5-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol at pH 7.5 and 3 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for RIT2

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Alternative Name: RIT2

Background: Rit and its neuron-specific homologue Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: GTP-binding protein Rit2, RIN, ROC2, Ras-like protein expressed in neurons, Ras-like without CAAX protein 2

Gene ID: 6014

UniProt: Q99578

Pathways: Neurotrophin Signaling Pathway