SMC1A antibody (AA 402-894)

Catalog No. ABIN264420

The Mouse Monoclonal anti-SMC1A antibody (Clone C2M) (ABIN264420) specifically detects SMC1A in WB and IF. The antibody is reactive with Human samples.

Quick Overview for SMC1A antibody (AA 402-894) (ABIN264420)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: C2M

Product Details anti-SMC1A Antibody

Binding Specificity: AA 402-894

Characteristics: Synonyms: SMC protein 1A, SMC-1A, SMC-1-alpha, SMC1L1, DXS423E, Structural maintenance ofchromosomes protein 1A, SB1.8

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinantprotein corresponding to amino acids 402-894 of human SMC1 and mouse myeloma cells. Genename: SMC1A

Application Details

Application Notes: Western Blot (1-5 μg/mL). Immunofluorescence(5-10 μg/mL). Positive Control: HeLa cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1A / SMC1

Background: Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus (2) protein in vivo.Synonyms: DXS423E, SB1.8, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1L1, Structural maintenance of chromosomes protein 1A

Gene ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance