S1PR4 antibody (C-Term) (FITC)

Catalog No. ABIN264452

The Mouse Monoclonal anti-S1PR4 antibody has been validated for WB and FACS. It is suitable to detect S1PR4 in samples from Human.

Quick Overview for S1PR4 antibody (C-Term) (FITC) (ABIN264452)

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S1PR4 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 1

Product Details anti-S1PR4 Antibody (FITC)

Binding Specificity: C-Term

Specificity: Recognizes EDG-6/S1P4 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: S1P receptor 4, S1PR4, EDG6, LPC1, SLP4, Sphingosine 1-phosphate receptor 4, Endothelialdifferentiation G-protein coupled receptor 6

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with Uniquesynthetic peptide derived from the C-terminal of the EDG6 (S1P4) protein and mousemyeloma cells.

Isotype: IgG1

Application Details

Application Notes: Western Blot. Flow Cytometry: Use 1 μg/10^6 cells. NOTE: Epitope for the antibody is the C-terminal of the EDG6 protein. The C-terminal is anintracellular region and cells need to be permeablized prior to staining with antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for S1PR4

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Alternative Name: S1PR4 / EDG6

Background: EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG6 (S1P4) receptor is the sphingosine 1-phosphate receptor-4 (S1P4).Synonyms: EDG-6, Endothelial differentiation G-protein coupled receptor 6, S1P receptor Edg-6, Sphingosine 1-phosphate receptor 4, Sphingosine 1-phosphate receptor Edg-6

Gene ID: 8698

UniProt: O95977