ATIC antibody

Catalog No. ABIN264511

This Mouse Monoclonal antibody specifically detects ATIC in WB and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Drosophila melanogaster and Frog.

Quick Overview for ATIC antibody (ABIN264511)

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Reactivity: Human, Mouse, Rat, Drosophila melanogaster, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATIC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F38 P7 H9

Product Details anti-ATIC Antibody

Specificity: This antibody detects Phosphoribosylaminoimidazolecarboxamide Formyltransferase (ATIC).

Characteristics: Synonyms: IMP cyclohydrolase, Inosinicase, IMP synthetase, Bifunctional purine biosynthesis proteinPURH

Purification: Affinity chromatography.

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the human ATIC protein and mouse myeloma Ag8563 cells. Sequence common in Frog, Fruit fly, Rat and Mouse.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1-2 μg/mL. Immunohistochemistry on Paraffin Sections (15 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1 x TBSwithTween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ATIC

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Alternative Name: ATIC / PURH

Background: The bifunctional purine biosynthesis protein PURH contains phosphoribosylaminoimidazole carboxamide formyltransferase, also designated AICAR transformylase, IMP cyclohydrolase or Inosinicase. AICAR plays an important role in purine biosynthesis, specifically in the production of nucleotides and IMP. Defects in ATIC, the gene encoding for this protein, can cause AICArebosuria, also designated AICA-ribosiduria, an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals with AICA-rebosuria accumulate AICA-ribotide, also designated ZMP, and its derivatives in erythrocytes and fibroblasts and also excrete very large amounts of AICA-riboside in the urine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are all symptoms of this disease.Synonyms: Bifunctional purine biosynthesis protein PURH, IMP cyclohydrolase, IMP synthetase, Inosinicase

Gene ID: 471

UniProt: P31939