CCT2 antibody (C-Term)

Catalog No. ABIN264513

This anti-CCT2 antibody is a Mouse Monoclonal antibody detecting CCT2 in WB and IHC (p). Suitable for Human, Mouse, Rat and Frog.

Quick Overview for CCT2 antibody (C-Term) (ABIN264513)

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Reactivity: Human, Mouse, Rat, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: F39 P7 F11

Product Details anti-CCT2 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes T-Complex Protein 1, beta subunit.

Characteristics: Synonyms: TCP1 Beta, CCT Beta, CCT-beta, CCTB, CCT2, 99D8.1, T-complex protein 1 subunit beta

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the Cterminal of the TCP-1β protein and mouse myelomaAg8563 cells. Sequence common in human, frog, mouse, rat.

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections: 1 - 5 μg/mL. Positive Control: Colorectal cancer tissue, Calu6 and HT29 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CCT2

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Alternative Name: CCT2 / TCP1 beta

Background: The protein TCP-1 (t-complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the Actins and Tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.Synonyms: 99D8.1, CCT-beta, CCTB, T-complex protein 1 subunit beta, TCP-1 beta

Molecular Weight: 57 kDa

Gene ID: 10576

UniProt: P78371